Isolation of bacteria contained in vermicompost with antifungal activity of chili anthracnose
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Abstract
The study aimed to isolate bacteria contained in vermicompost with the antifungal activity of chili anthracnose. The causative agent of chili anthracnose was isolated and identified. Subsequently, the bacteria were isolated from vermicompost, their antifungal activities were investigated by the dual culture method and identified by morphology. The results found that the fungal pathogen of chili anthracnose was isolated and identified as Colletotrichum siamense by morphological characteristics and sequence analysis of ITS (Internal Transcribed Spacer), GAPDH (Glyceraldehyde-3-Phosphate Dehydrogenase), and TUB2 (Beta-tubulin2) gene. Twenty bacterial isolates were isolated from vermicompost. Among these isolates, 4 bacterial isolates revealed antifungal activity against C. siamense including B5, B3, A6, and A5 with inhibition percentages as 47.78±3.85%, 34.45±10.72%, 34.44±1.93% and 26.67±3.34%, respectively. These bacterial isolates were presumptively identified by morphology revealing that all bacteria isolated were classified as the endospore-forming gram-positive rod.
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