Parents-child analysis with 44 single nucleotide polymorphisms (SNPs) in Thai individuals
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Abstract
The aim of research is to determine the parent-child relationship based upon 44 SNPs markers using statistic values of paternity index (PI). Samples of this study were in two groups: true parent-child (n= 38 families) and unrelated groups (n=38 families). The results showed that the true parent-child group shared 44 loci (38 families: 100%), and the unrelated groups had loci mismatch between 2 to 13 loci. The four mismatch loci were the highest number of families (10 families: 26.32%). The maximum and minimum of PI values were 1.0120x107 and 83.9371 were among the true parent-child groups. The unrelated group's maximum and minimum PI values were 5.0065x104 and 7.0750, respectively. The lower limit of the distribution of the true parent-child intersected with the upper limit of the distribution of the unrelated between the values of 83.9371 to 5.0065x104. In this range of values, the distribution of the PI values could not be used to determine the relationship and therefore was recognized as a “gray zone”, whereas the non-relationship PI is from the calculation of mismatch elimination. This research showed that the difference between true parent-child and unrelated groups using 44 SNPs could help to predict human relationships and can be further applied in other relationships.
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