Embryo Axis Culture of Hopea thorelii Pierre, a Critically Endangered Species
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Abstract
Hopea thorelii Pierre is a tree in the family Dipterocarpaceae. It is less distributed and classified as a critically endangered species. It is usually propagated from seeds, which is limited. Plant tissue culture is an important technique to provide a rapid plant propagation. Hence, this research aimed to study the effects of N6-benzyladenine (BA), naphthaleneacetic acid (NAA) and indole-3-butyric acid (IBA) on growth of embryo axis of Hopea thorelii Pierre in vitro. Embryo axis of three months old fruits collected from Ubon Ratchathani Zoo were cultured on MS medium (Murashige and Skoog) contained combinations of 0, 0.1 and 1 mg/l IBA and 0, 1, 2, 4, 6 and 8 mg/l BA and MS medium contained combinations of 0, 0.1 and 1 mg/l NAA and 0, 1, 2, 4, 6 and 8 mg/l BA for 8 weeks. The results showed that MS medium supplemented with IBA:BA and NAA:BA at 0.1:4 and 0:1 mg/l led to the highest number of shoots (1.40 and 1.20 shoots/embryo, respectively). These results are basic information which might be useful for the next research of H. thorelii Pierre tissue culture.
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