Bioaccumulation of zinc by Portunus pelagicus: Nuclear application techniques that use radiotracer 65Zn to study influence of concentration of Zn in seawater

Authors

  • Ikhsan Budi Wahyono Research Center for Environmental and Clean Technology, National Research and Innovation Agency (BRIN), Puspitek Serpong, Tangerang Selatan 15314, Indonesia
  • Muslim Department of Oceanography, Faculty of Fisheries and Marine Science, Diponegoro University, Semarang, 50271 Indonesia
  • Heny Suseno Research Center for Radioisotope Technology, Radiopharmaceuticals, and Biodosimetry, National Research and Innovation Agency (BRIN), Puspitek Serpong, Tangerang Selatan 15314, Indonesia
  • Chrisna Adhi Suryono Department of Marine Science, Faculty of Fisheries and Marine Science, Diponegoro University, Semarang, 50271 Indonesia
  • Anung Pujiyanto Research Center for Radioisotope Technology, Radiopharmaceuticals, and Biodosimetry, National Research and Innovation Agency (BRIN), Puspitek Serpong, Tangerang Selatan 15314, Indonesia

DOI:

https://doi.org/10.33175/mtr.2024.266903

Keywords:

Bioaccumulation, Depuration, Zinc, Portunus pelagicus, Pollution

Abstract

Zinc (Zn) is an essential trace metal, but also a potential toxicant to aquatic organisms. Zinc is one of the toxicants from various industrial and domestic activities whose waste is directly discharged into water. One of the marine organisms used as an indicator of pollution is the blue swimming crab (Portunus pelagicus). Bioaccumulation studies of Zn in Portunus pelagicus from Jakarta Bay were conducted in a radiotracer laboratory. Laboratory experiments were done to study the uptake and release of 65Zn radioisotope in Portunus pelagicus. The experiments conducted were biota collection, acclimatization, bioaccumulation, and elimination. This study was to determine the relationship between the amount of 65Zn released into the aquatic environment and its effect on Zn bioaccumulation ability and radiation dose received by Portunus pelagicus. Biokinetics parameters, such as concentration factors CF, uptake rate constant ku, elimination rate constant ke, and concentration factor steady state (CFss) were investigated. The results showed that an increase in concentration would decrease the rate of absorption and rate of elimination, and that the concentration factor CF values ​​varied from 7.82 - 60.62 ml.g-1 in Portunus pelagicus. The concentration factor at steady-state Zn was 25.55-59.47 ml.g–1, which is influenced by the concentration after 6 days of exposure. The depuration rate was observed to be high, with a value of retention 34.33 - 78.88 % of 65Zn absorbed by Portunus pelagicus, which was absent 7 days after exposure.

Highlights

  • Laboratory experiments lasting 14 days showed that Portunus pelagicus has the ability to accumulate the heavy metal zinc (Zn) from the environment and release it back into the water
  • The highest bioaccumulation kinetics of zinc (Zn) metal in Portunus pelagicus during the 7-day experiment occurred at a contaminant concentration of 0.5 ppm with an absorption constant (ku) value of 0.7838 d-1 and a release constant value (ke) of 0.0892 d-1
  • Portunus pelagicus concentration factor (CF) has a moderate value and the smallest uptake constant (ku) and elimination constant (ke) value compared to other biota

References

Al-Mustawa, M., Budiawan, B., & Suseno, H. (2022). The effect of zinc speciation and its concentration on bioaccumulation in pomfret (Colossoma macropomum) and sepat fish (Trichogaster Trichopterus). Trends in Sciences, 20(2), 4047. https://doi.org/10.48048/tis.2023.4047

Asante, F., Agbeko, E., Addae, G., & Quainoo, A. K. (2014). Bioaccumulation of heavy metals in water, sediments and tissues of some selected fishes from the Red Volta, Nangodi in the Upper East Region of Ghana. Current Journal of Applied Science and Technology, 4(4), 594-603. https://doi.org/10.9734/BJAST/2014/5389

Authman, M. M. N., Zaki, M. S., Khallaf, E. A., & Abbas, H. H. (2015). Use of fish as bio-indicator of the effects of heavy metals pollution. Journal of Aquaculture Research & Development, 6(4), 1-13. https://doi.org/10.4172/2155-9546.1000328

Azizi, G., Akodad, M., Baghour, M., Layachi, M., & Moumen, A. (2018). The use of Mytilus spp. mussels as bioindicators of heavy metal pollution in the coastal environment: A review. Journal of Materials and Environmental Sciences, 9(4), 1170-1181.

Bartzatt, R. (2017). Neurological impact of zinc excess and deficiency in vivo. European Journal of Nutrition & Food Safety, 7(3), 155-160. https://doi.org/10.9734/ejnfs/2017/35783

Belivermiş, M., Swarzenski, P. W., Oberhänsli, F., Melvin, S. D., & Metian, M. (2020). Effects of variable deoxygenation on trace element bioaccumulation and resulting metabolome profiles in the blue mussel (Mytilus edulis). Chemosphere, 250, 126314. https://doi.org/10.1016/j.chemosphere.2020.126314

Bonsignore, M., Salvagio Manta, D., Mirto, S., Quinci, E. M., Ape, F., Montalto, V., Gristina, M., Traina, A., & Sprovieri, M. (2018). Bioaccumulation of heavy metals in fish, crustaceans, molluscs and echinoderms from the Tuscany coast. Ecotoxicology and Environmental Safety, 162, 554-562. https://doi.org/https://doi.org/10.1016/j.ecoenv.2018.07.044

Briffa, J., Sinagra, E., & Blundell, R. (2020). Heavy metal pollution in the environment and their toxicological effects on humans. Heliyon, 6(9), e04691. https://doi.org/10.1016/j.heliyon.2020.e04691

Budiawan, B., Suseno, H., Afriani, F., & Prihatiningsih, W. R. (2021). Bioaccumulation and retention kinetics of trace elements in the horse mussels Modiolus micropterus exposed to different environmental conditions. Environmental Toxicology and Pharmacology, 87, 103692. https://doi.org/10.1016/j.etap.2021.103692

Carvalho, F. P. (2018). Radionuclide concentration processes in marine organisms: A comprehensive review. Journal of Environmental Radioactivity, 186, 124-130. https://doi.org/10.1016/j.jenvrad.2017.11.002

Foyle, K. L., Hess, S., Powell, M. D., & Herbert, N. A. (2020). What is gill health and what is its role in marine finfish aquaculture in the face of a changing climate? Frontiers in Marine Science, 7, 400. https://doi.org/10.3389/fmars.2020.00400

Herrero, A., Thompson, K. D., Ashby, A., Rodger, H. D., & Dagleish, M. P. (2018). Complex gill disease: an emerging syndrome in farmed Atlantic salmon (Salmo salar L.). Journal of Comparative Pathology, 163, 23-28. https://doi.org/10.1016/j.jcpa.2018.07.004

Jayawiguna, M. H., Mulyono, M., Nugraha, E., Prayitno, H., & Basith, A. (2017). Biology aspect of blue swimming crabs (Portunus pelagicus) in Jakarta Bay Waters, Indonesia. Australian Journal of Basic and Applied Sciences, 11(13), 63-67.

Karanjkar, D. M., & Deshpande, V. Y. (2020). Zinc induced histopathological alternations in gill, liver, and kidney of freshwater fish Gonoproktopterus kolus (Sykes). Advances in Zoology and Botany, 8(5), 400-407. https://doi.org/10.13189/azb.2020.080504

Kucukosmanoglu, A. G., & Filazi, A. (2020). Investigation of the metal pollution sources in Lake Mogan, Ankara, Turkey. Biological Trace Element Research, 198(1), 269-282. https://doi.org/10.1007/s12011-020-02031-z

Kunzmann, A., Arifin, Z., & Baum, G. (2018). Pollution of coastal areas of Jakarta Bay: Water quality and biological responses. Marine Research in Indonesia, 43(1), 37-51. https://doi.org/10.14203/mri.v43i1.299

Kuranchie-Mensah, H., Pouil, S., Teyssié, J. L., Oberhänsli, F., Warnau, M., & Metian, M. (2018). Allometric relationship in the bioaccumulation of radionuclides (134Cs & 241Am) and delineation of contamination pathways (food and seawater) in bloody cockle Anadara senilis using radiotracer techniques. Journal of Environmental Radioactivity, 192, 448-453. https://doi.org/10.1016/j.jenvrad.2018.07.018

Lee, J., Birch, G., Cresswell, T., Payne, T. E., & Simpson, S. L. (2014). Bioaccumulation of 65Zn by the Sydney rock oyster (Saccostrea glomerata) from dissolved and particulate phases (pp. 14-17). In Proceedings of the SETAC Australasia. Adelaide, Australasia.

Malik, D. S., & Maurya, P. K. (2014). Heavy metal concentration in water, sediment, and tissues of fish species (Heteropneustis fossilis and Puntius ticto) from Kali River, India. Toxicological & Environmental Chemistry, 96(8), 1195-1206. https://doi.org/10.1080/02772248.2015.1015296

McDonald, S., Cresswell, T., & Hassell, K. (2020). Bioaccumulation kinetics of cadmium and zinc in the freshwater decapod crustacean Paratya australiensis following multiple pulse exposures. Science of the Total Environment, 720, 137609. https://doi.org/10.1016/j.scitotenv.2020.137609

Moniruzzaman, M., Kumar, S., Das, D., Sarbajna, A., & Chakraborty, S. B. (2020). Enzymatic, nonenzymatic antioxidants and glucose metabolism enzymes response differently against metal stress in muscles of three fish species depending on different feeding niche. Ecotoxicology and Environmental Safety, 202, 110954. https://doi.org/10.1016/j.ecoenv.2020.110954

Ngo, H. T. T., Gerstmann, S., & Frank, H. (2011). Subchronic effects of environment-like cadmium levels on the bivalve Anodonta anatina (Linnaeus 1758): III. Effects on carbonic anhydrase activity in relation to calcium metabolism. Toxicological & Environmental Chemistry, 93(9), 1815-1825. https://doi.org/10.1080/02772240802503619

Nugraha, B., Triharyuni, S., Suleman, P. S., & Hartati, S. T. (2020). Fisheries status and habitat conditions in the Waters of Jakarta Bay. Jakarta Research Journal, 13(1), 17-28. https://doi.org/10.37439/jurnaldrd.v13i1.17

Popek, E. P. (2017). Sampling and analysis of environmental chemical pollutants: A complete guide. Elsevier. https://doi.org/10.1016/C2014-0-03819-1

Pouil, S., Clausing, R. J., Metian, M., Bustamante, P., & Dechraoui-Bottein, M. Y. (2018). A study of the influence of brevetoxin exposure on trace element bioaccumulation in the blue mussel Mytilus edulis. Journal of Environmental Radioactivity, 192, 250-256. https://doi.org/10.1016/j.jenvrad.2018.06.008

Prihatiningsih, W. R., Suseno, H., Zamani, N. P., & Soedharma, D. (2016). Bioaccumulation and retention kinetics of cesium in the Milkfish Chanos chanos from Jakarta Bay. Marine Pollution Bulletin, 110(2), 647-653. https://doi.org/10.1016/j.marpolbul.2016.04.058

Riani, E., Johari, H. S., & Cordova, M. R. (2017). Contamination of Pb and Cd in Milkfish (Chanos chanos) Cultured in the Seribu Islands. Marine Tropical Science and Technology Journal, 9(1), 235-246. https://doi.org/10.29244/jitkt.v9i1.17938

Riani, E., Sudarso, Y., & Cordova, M. R. (2014). Heavy metals effect on unviable larvae of Dicrotendipes simpsoni (Diptera: Chironomidae), a case study from Saguling Dam, Indonesia. Aquaculture, Aquarium, Conservation & Legislation, 7(2), 76-84.

Sabnavis, M., Jagasheth, U. H., Avachat, H., & Mishra, M. (2018). Zinc industry: The unsung metal of the economy. CARE Ratings: Professional Risk Opinion, Report.

Squadrone, S., Brizio, P., Stella, C., Mantia, M., Favaro, L., Biancani, B., Gridelli, S., Da Rugna, C., & Abete, M. C. (2020). Differential bioaccumulation of trace elements and rare earth elements in the muscle, kidneys, and liver of the invasive Indo-Pacific Lionfish (Pterois spp.) from Cuba. Biological Trace Element Research, 196, 262-271. https://doi.org/10.1007/s12011-019-01918-w

Truchet, D. M., Buzzi, N. S., Simonetti, P., & Marcovecchio, J. E. (2020). Uptake and detoxification of trace metals in estuarine crabs: Insights into the role of metallothioneins. Environmental Science and Pollution Research, 27, 31905-31917. https://doi.org/10.1007/s11356-020-09335-6

Yu, D., Peng, X., Ji, C., Li, F., & Wu, H. (2020). Metal pollution and its biological effects in swimming crab Portunus trituberculatus by NMR-based metabolomics. Marine Pollution Bulletin, 157, 111307. https://doi.org/10.1016/j.marpolbul.2020.111307

Zuraida, R., Rahardiawan, R., Permanawati, Y., Adhirana, I., Ibrahim, A., Nurdin, N., & Permana, H. (2018). Late holocene heavy metals record of Jakarta Bay Sediments. Bulletin of the Marine Geology, 33(2), 119-130. https://doi.org/10.32693/bomg.33.2.2018.569

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2023-09-14

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Vol. 6 No. 1 (2024): January - March

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