Genetic Analysis of Stellantchasmus falcatus on HAT-RAPD Technique

Abstract

Twenty random primers were selected to detect the molecular marker in Stellantchasmus falcatus with other four heterophyid flukes(Haplorchis taichui, Centrocestus caninus, Metagonimus yokogawai and Haplorchoides sp.) and liver fluke (Opisthorchis viverrini) by using high annealing temperature random amplification of polymorphic DNA(HAT-RAPD) technique. In total, HAT-RAPD had generated 354 genetic markers of which only 0.56% was shared by the six species studied and separated the six speciesstudied into two major clusters: S. falcatus, H. taichui, C. caninusand M. yokogawai were grouped together; the second cluster is Haplorchoides sp. with O. viverrini as outgroup.