Bulblets Induction of Lilium longiflorum Hybrid var. Formolongo In Vitro Culture

Abstract

The multiplication of the economic flower, Lilium longiflorum Hybrid var. Formolongo by in vitro culture induction in suitable medium was investigated. The bulb scales of L.longiflorum Hybrid var. Formolongo were sterilized with Clorox on various concentration 10%, 20%, 30% and 40% for 20 minutes, then cultured on hormone free MS medium for 1 week. The result showed that Clorox 30% could sterile bulb scales about 62%. The explants were transferred on MS medium with BA and NAA (0, 0.5, 0.75, 1.0, 1.5 and 2.0 mg/l respectively) for 8 weeks. The MS medium with 0.75 mg/l BA and 1.0 mg/l NAA could highly induced multiple shoot formation. After that cutting shoots into small segments were cultured on hormone free MS medium for 6 weeks and got small single shoots (6 mm.). The small single shoots were transferred onto MS medium supplemented with 30, 60, 90 and 120 g/l sucrose respectively for 8 weeks. Bulblets had the biggest diameter (14 mm.) on MS medium with 60 g/l sucrose. Therefore, in this study MS medium with 60g/l sucrose was suitable medium for bulblets induction of L. longiflorum Hybrid var. Formolong in culture.