Effect of Plant Growth Regulator on Plant Tissue Culture of Dendrobium hercoglossum Rchb.f.

Abstract

Tissue culture of Dendrobium hercglossum Rchb.f. from leaf tip, node and shoot tip was investifgated. These explants were cultured on Murashige and Skoog (MS) medium supplemented with benzyladenine (BA) and kinetin (KN) at 5 different concentrations (0.5, 1.0, 2.0, 4.0 and 8.0 mg/L) and 2,4-Dichlorophenoxyacetic acid (2,4-D) at 4 different concentrations (0.5, 1.5, 1.5 and 2.0 mg/L). All cultures were incubated at 25 2 under cool white fluorescent light at intensity of 2000 lux for a 16-h and maintained at 12 weeks. Shoot tip developed into plantets on MS medium supplemented with different types and concentrations of plant growth regulators. The result showed no significant difference in roots, leaves, fresh weight and shoots whereas significant difference was obtained in shoot length. MS medium supplemented with 1.0 mg/L BA gave the highest average number of shoot length (5.83 cm/plantlet), leaves (5.50 leaves/plantlet) and fresh weight (0.19 gram/plantlet), 0.5 mg/L 2,4-D and 0.5 mg/L KN gave the highest average number of roofs (6.33 roots/plantlet) and shoots (4.67 shoots/plantlet), respectively.