Effects of Aqueous Extracts of Siamese Cassia’s Root on the Death and the Tegumental Surface of the Trematode Using Scanning Electron

Abstract

In vitro effects of aqueous extracts of Siamese cassia’s Root (Cassia siamea Britt.) were studied on tegumental surface of trematode (Stellantchasmus falcatus). Findings an appropriated concentration and time which effect on trematode were investigated by light microscope. The worms were examined the death with aqueous extract 6.25%, 12.5%, 25% and 50% in Tyrode’s solution at 1, 6, 12 and 24 hours respectively. Worms in Tyrode's solution were served as controls. The result showed that extract of 6.25%, 12.5% and 25% can kill all the worms within 6 hours, while 50% extract concentration can kill worms 92.5 % within 1 hour. The SEM (Scanning Electron Microscopy) observation of the death trematode focused mainly on the tegumental surface of the worm’s body. It was found that the trematode in the extract of 6.25% solution showed loss of the scales at 6 hours and curving at the edge of scales at 12 hours. The trematode in the extract of 12.5% solution showed loss of the scales and curving at the edge of scales at 6 hours. Whereas the trematode in the extract of 25% and 50% solution had the same variations of the tegument as the trematode in the extract of 12.5% but more apparently scale loosing at 1 hour. It was also found that rupturing and the tegument tearing off from a tegumental surface were evident at 12 hours. This findings suggest that Siamese cassia’s root is an effective anthelminthic drug.